Electrophysiology - 
recording of synaptic transmission and  plasticity

Fields of research:

Evaluation of compound actions on (glutamatergic) synaptic transmission

Detection of side effects of putative therapeutics

Evaluation of processes underlying learning and memory and mechanisms relevant for Alzheimer's disease

Metabotropic receptors, Neuropeptides, Neuromodulators

Long-term potentiation (LTP), long-term depression (LTD).  First laboratory with systematic recording of LTP over 10 hours in vitro

Methods of research:

Extracellular long-term recordings of field potentials (field-EPSP, population spike, paired pulse inhibition and facilitation) are performed in the CA1 or dentate gyrus region of freshly isolated slices from adult rats or organotypic slice cultures  (400 µm thick). The slices are kept in either surface or submerged-type chamber superfused with oxygenated ACSF. Lacquer-coated stainless steel monopolar stimulation electrode and a glass recording-electrode (filled with ACSF) are placed in the stratum radiatum of the CA1 region to record field excitatory postsynaptic potentials (fEPSPs) or population spikes . Input-output (I/O) curves are generated to define appropriate test stimulus strength.  Once stable responses are obtained for at least 60 min, Depending on the aim of the study various forms of short-term and lng-term potentiation can be induced by appropriate tetanization protocols (normally consisting of 100 pulses at 100 Hz).

For further information contact:

Dr. Ulrich Schröder
phone: +49(391)6263781 or 6117208
schroede@ifn-magdeburg.de 

Prof. Klaus Reymann
phone: +49(391)6263437
fax +49(391)6263438
reymann@fan-neuroscience.com  

Recent publications:

Fedorov, N.B., and Reymann, K.G.: Simultaneous local pressure microejection of excitatory amino acids and field potential recording with a single micropipette in the hippocampal slice. J. Neurosci. Meth., 1993, 50, 83-90

Platt, B., and Reymann, K.G.: Inhibition of TEA-induced LTP by Aluminium. Exp. Neurol., 1996, 141, 240-247

Brown, R.E. and Reymann, K.G.: Histamine H3 receptor mediated depression of synaptic transmission in the dentate gyrus of the rat in vitro. J. Physiol., 1996, 496, 175-184

Fedorov, N.B., Pasinelli, P., Oestreicher, A.B., DeGraan, P.N.E., and Reymann, K.G.: Antibodies to postsynaptic PKC substrate neurogranin prevent long-term potentiation in hippocampal CA1 neurons. Eur. J. Neurosci., 1995, 7, 819-822

Wilsch, V.W., Behnisch, T., Jäger, T., Reymann, K.G. and Balschun, D.: When are class I metabotropic glutamate receptors necessary for LTP? J. Neurosci. 1998, 18, 6071-6080

Szinyei, C., Behnisch, T. and Reymann, K.G.: Long-term potentiation in rat CA1 neurons requires post-synaptic calcium induced calcium release. J. Physiology 1999, 1999, 516, 855-868