Mitochondrial function testing |
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Fields of research:Anoxia / reoxygenation Ion transport in mitochondria Permeability of the mitochondrial membrane
Methods of research:Measurement of oxygen consumption Determination of mitochondrial membrane potential Determination of enzyme activities Characterization of cytochrome c release Swelling experiments Mitochondria from different tissues (brain, heart,
liver) are prepared from 220-240 g male Wistar rats in ice-cold medium by
using a standard procedure. Oxygen uptake of the mitochondria is measured
at 30°C in a thermostat-controlled chamber equipped with a Clark-type and
a TPP+-sensitive electrode. The mitochondrial membrane potential is
calculated from the extramitochondrial TPP+-concentration. Respiration and
membrane potential characterise the functional intactness of the
mitochondria. During Hypoxia the mitochondrial membrane potential collapse
partially restoring after reoxygenation indicating damage of mitochondria
by hypoxia/reoxygenation. Respiration and membrane potential measurments
are also used for studying ion transports through the mitochondrial
membrane. Determination of the permeability of the mitochondrial membrane
and measurements of enzyme activities are additional components of our
spectrum of methods. |
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For further information please contact:
PD Dr. rer. nat. Lorenz Schild
Recent Publications:Schild, L., Reinheckel, T., Wiswedel, I., Augustin, W.: Short term impairment of energy production in isolated rat liver mitochondria by hypoxia/reoxygenation: Involvement of oxidative protein modification. Biochem. J. 328, 205-210 (1997) Schild, L., Plumeyer, F., Reinheckel, T.; Augustin, W.: Micromolar calcium prevents isolated rat liver mitochondria from anoxia-reoxygenation injury. Biochem. Mol. Biol. Int. 43, 35-45 (1997) Schild, L., Gellerich F.N.: Effect of the extramitochondrial adenine nucleotide pool size on oxidative phosphorylation in isolated rat liver mitochondria. Eur. J. Biochem. 252, 508-512 (1998) Schild, L., Matthias, R., Stanarius, A., Wolf, G., Augustin, W., Halangk, W.: Induction of permeability transition in pancreactic mitochondria by cerulein in rat. Biol. Cell. Biochem. 195, 191-7 (1999) Schild, L., Keilhoff, G., Augustin, W., Reiser, G., Striggow, F.
Distinct Ca2+ thresholds determine cytochrome c release or permeability
transition pore opening in brain mitochondria. FASEB J.2001, online |
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